af1924 foxa2 rabbit  (R&D Systems)

Journal: Stem cell research

Article Title: Generation of two induced pluripotent stem cell lines from breast cancer patients carrying BRCA2 variants

doi: 10.1016/j.scr.2023.103219

Figure Lengend Snippet: Reagents details

Article Snippet: Table 2: Antibodies used for immunocytochemistry/flow-cytometry Antibody Dilution Company Cat # RRID Pluripotency Markers Rabbit Anti-Nanog 1:100 Proteintech Cat# 14295-1-AP RRID: AB_1607719 Mouse IgG 2b κOct3/4 antibody 1:100 Santa Cruz Biotechnology Cat# sc-5279 RRID: AB_628051 Goat IgG anti Sox2 1:100 R and D Systems Cat# AF2018 RRID: AB_355110 Ectoderm Markers Goat Anti-Otx2 1:200 R and D Systems Cat# AF1979 RRID: AB_2157172 Rabbit Anti-Pax6 1:200 Thermo Fisher Scientific Cat# 42-6600 RRID: AB_2533534 Endoderm Markers Goat Anti-Sox17 1:200 R and D Systems Cat# AF1924 RRID: AB_355060 Rabbit Anti-Foxa2 1:250 Thermo Fisher Scientific Cat# 701698 RRID: AB_2576439 Mesoderm Markers Goat Anti-Brachyury 1:200 R and D Systems Cat# AF2085 RRID: AB_2200235 Rabbit Anti-Tbx6 1:200 Thermo Fisher Scientific Cat# PA5-35102 RRID: AB_2552412 Secondary antibodies Alexa Fluor 647 Goat Anti-Mouse IgG 2b 1:250 Thermo Fisher Scientific Cat# A-21242 RRID: AB_2535811 Alexa Fluor 555 Goat Anti-Rabbit IgG (H+L) 1:500 Thermo Fisher Scientific Cat# A-21428 RRID: AB_141784 Alexa Fluor 488 Donkey Anti-Goat IgG 1:1000 Thermo Fisher Scientific Cat# A-11055 RRID: AB_2534102 Primers Target Size of band Forward/Reverse primer (5′-3′) Sendai virus plasmid (qPCR) Sendai virus genome 181bp Mr042698800_mr (Thermo Fisher Scientific) Pluripotency Markers (qPCR) NANOG 109bp Hs02387400_g1 (Thermo Fisher Scientific) House-Keeping Genes (qPCR) POU5F1 77bp Hs00999632_g1 (Thermo Fisher Scientific) Pluripotency Markers (qPCR) SOX2 86bp Hs04234836_s1 (Thermo Fisher Scientific) House-Keeping Genes (qPCR) GAPDH 157bp Hs02786624_g1 (Thermo Fisher Scientific) Genotyping BRCA2 c.8167G>C 350bp Forward: 5’-TCACTTTTAGATATGATACG-3’ Reverse: 5’-TTCTGGGGCTTCAAGAGGTG-3’ Genotyping BRCA2 c.1583del 350bp Forward: 5’-AGGAAACAGTGGTAAATAAG-3’ Reverse: 5’-TGCATTCTTCAAAGCTACAG-3’ Open in a separate window Reagents details.

Techniques: Immunocytochemistry

Journal: Stem cell research

Article Title: Generation of two induced pluripotent stem cell lines from breast cancer patients carrying BRCA2 variants

doi: 10.1016/j.scr.2023.103219

Figure Lengend Snippet: Characterization and validation

Article Snippet: Table 2: Antibodies used for immunocytochemistry/flow-cytometry Antibody Dilution Company Cat # RRID Pluripotency Markers Rabbit Anti-Nanog 1:100 Proteintech Cat# 14295-1-AP RRID: AB_1607719 Mouse IgG 2b κOct3/4 antibody 1:100 Santa Cruz Biotechnology Cat# sc-5279 RRID: AB_628051 Goat IgG anti Sox2 1:100 R and D Systems Cat# AF2018 RRID: AB_355110 Ectoderm Markers Goat Anti-Otx2 1:200 R and D Systems Cat# AF1979 RRID: AB_2157172 Rabbit Anti-Pax6 1:200 Thermo Fisher Scientific Cat# 42-6600 RRID: AB_2533534 Endoderm Markers Goat Anti-Sox17 1:200 R and D Systems Cat# AF1924 RRID: AB_355060 Rabbit Anti-Foxa2 1:250 Thermo Fisher Scientific Cat# 701698 RRID: AB_2576439 Mesoderm Markers Goat Anti-Brachyury 1:200 R and D Systems Cat# AF2085 RRID: AB_2200235 Rabbit Anti-Tbx6 1:200 Thermo Fisher Scientific Cat# PA5-35102 RRID: AB_2552412 Secondary antibodies Alexa Fluor 647 Goat Anti-Mouse IgG 2b 1:250 Thermo Fisher Scientific Cat# A-21242 RRID: AB_2535811 Alexa Fluor 555 Goat Anti-Rabbit IgG (H+L) 1:500 Thermo Fisher Scientific Cat# A-21428 RRID: AB_141784 Alexa Fluor 488 Donkey Anti-Goat IgG 1:1000 Thermo Fisher Scientific Cat# A-11055 RRID: AB_2534102 Primers Target Size of band Forward/Reverse primer (5′-3′) Sendai virus plasmid (qPCR) Sendai virus genome 181bp Mr042698800_mr (Thermo Fisher Scientific) Pluripotency Markers (qPCR) NANOG 109bp Hs02387400_g1 (Thermo Fisher Scientific) House-Keeping Genes (qPCR) POU5F1 77bp Hs00999632_g1 (Thermo Fisher Scientific) Pluripotency Markers (qPCR) SOX2 86bp Hs04234836_s1 (Thermo Fisher Scientific) House-Keeping Genes (qPCR) GAPDH 157bp Hs02786624_g1 (Thermo Fisher Scientific) Genotyping BRCA2 c.8167G>C 350bp Forward: 5’-TCACTTTTAGATATGATACG-3’ Reverse: 5’-TTCTGGGGCTTCAAGAGGTG-3’ Genotyping BRCA2 c.1583del 350bp Forward: 5’-AGGAAACAGTGGTAAATAAG-3’ Reverse: 5’-TGCATTCTTCAAAGCTACAG-3’ Open in a separate window Reagents details.

Techniques: Immunofluorescence, Staining, Expressing, Mutagenesis, Sequencing, Southern Blot, Reverse Transcription Polymerase Chain Reaction